Regulatory T cells in experimental allergic encephalomyelitis. II. T cells functionally antagonistic to encephalitogenic MBP-specific T cells show persistent expression of FasL
✍ Scribed by Deming Sun; John N. Whitaker; Darcy B. Wilson
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 186 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Naive LEW rats and animals that have recovered from active or adoptive EAE contain a subset of T cells that inhibit EAE in secondary recipients and are lytic for MBP-reactive T cell lines in culture. Here we explore various features of these regulatory T cell populations (RTC) with respect to (1) their frequency in animals following immunization with syngeneic MBP-reactive cell lines, (2) their ability to inhibit proliferative responses by MBP-reactive cell lines in culture, (3) their ability to lyse MBP-specific target cells, and especially (4) their prolonged expression of high levels of FasL following activation in culture correlating with their lytic effects on A20, a FasLsensitive mouse lymphoma cell line. Inhibition studies indicate that mAbs specific for MHC class I and MHC class II molecules inhibit lysis of syngeneic MBPreactive target T cells, soluble Fas protein shows some inhibition, but none of these agents inhibits the lytic effects of activated RTC on the A20 cell line. J.