Regulation of Tyrosine Hydroxylase and Dopamine β-Hydroxylase mRNA Levels in Rat Adrenals by a Single and Repeated Immobilization Stress
✍ Scribed by Anne McMahon; Richard Kvetňansk; Koki Fukuhara; Virginia K. Weise; Irwin J. Kopin; Esther L. Sabban
- Book ID
- 111177588
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 887 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0022-3042
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## Abstract A number of cDNA clones for rat dopamine β‐hydroxylase (DBH) were isolated from a rat pheochromocytoma tumor cDNA library. The 2445 nucleotide sequence revealed a single open reading frame of 1860 nucleotides and a 3′ untranslated region containing two polyadenylation addition signals.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of catecholamines in neural tissues and adrenal medulla. To study the expression of the TH gene and its regulation in adult and developing neural tissues, we have synthesized an oligodeoxyribonucleotide probe (oligomer) that is s