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Regulation of transcription of theSaccharomyces cerevisiae CYC1gene: Identification of a DNA region involved in heme control

✍ Scribed by Rosmarie Gudenus; Andrew Spence; Andreas Hartig; Michael Smith; Helmut Ruis

Publisher: Springer-Verlag
Year: 1984
Tongue: English
Weight: 483 KB
Volume: 8
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00405431

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✦ Synopsis

A Saccharornyces cerevisiae mutant (hem1 cycl-1) was transformed with plasmids bearing a chromosomal centromer (CEN3) and a 2 /lm DNA replication origin. In one of the plasmids a functional CYC1 gene was present, in a second plasmid an XhoI fragment located between bases -245 and -678 upstream from the translation initiation codon had been deleted, in a third plasmid this region had been inverted. Results of hybridization experiments carried out with mRNA isolated from heme-deficient and heme-containing transformants indicated that heme controls transcription of the CYC1 gene and that DNA sequences located within the upstream XhoI fragment are involved in activation of the gene by heme.

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