𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Regulation of theADE2gene fromSaccharomyces cerevisiae

✍ Scribed by Agathe Stotz; Peter P. Müller; Patrick Linder

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
754 KB
Volume
24
Category
Article
ISSN
0172-8083

No coin nor oath required. For personal study only.

✦ Synopsis

Regulation of ADE2 gene expression was investigated in the yeast S. cerevisiae using translational fusions between this gene and the lacZ gene from E. coli. Expression was repressed in the presence of adenine and slightly increased under amino-acid starvation conditions. The promoters of the ADE2 gene, and of other genes involved in adenine biosynthesis, contain the hexanucleotide sequence TGACTC. A search for the hexanucleotide TGACTC in yeast promoter sequences revealed that many genes not related to amino-acid biosynthesis contain such sequences. We show here that these elements play a crucial role in ADE2 regulation since mutations in two such elements drastically reduced gene expression. Maximal expression required the transcriptional activators Basl, Bas2 and Gcn4, whereas Yap1 had only minor effects.

📜 SIMILAR VOLUMES

Control of the expression of theADE2gene
Control of the expression of theADE2gene of the yeastSaccharomyces cerevisiae
✍ Alma Gedvilaite; Kęstutis Sasnauskas 📂 Article 📅 1994 🏛 Springer-Verlag 🌐 English ⚖ 654 KB

The ADE2 gene encodes AIR-carboxylase which catalyzes the sixth step of the purine biosynthetic pathway in Saccharomyces cerevisiae. We have analyzed the effect of deletions in the promoter region of this gene on the expression of the enzyme using a fusion of the ADE2 gene promoter to the bacterial

Identification of the gene encoding scHe
Identification of the gene encoding scHelI, a DNA helicase fromSaccharomyces cerevisiae
✍ Bean, Daniel W.; Matson, Steven W. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 141 KB 👁 2 views

The gene encoding scHelI, a previously characterized DNA helicase from Saccharomyces cerevisiae, has been identified as YER176w, an open reading frame on chromosome V. The gene has been named HEL1 to indicate the DNA helicase activity of the gene product. HEL1 was identified by screening a gt11 yeas

Properties of β-glucan synthetase fromSa
Properties of β-glucan synthetase fromSaccharomyces cerevisiae
✍ E. López-Romero; J. Ruiz-Herrera 📂 Article 📅 1978 🏛 Springer Netherlands 🌐 English ⚖ 558 KB

Properties of beta-glucan synthetase from S. cerevisiae were studied. The enzyme exhibited optimal activity at pH 6.7 and 24 C. Km for UDP-glucose was 0.12 mM. Addition of Mg++ or Mn++ stimulated its activity by 60% and 21% respectively. High concentrations of EDTA and hydroxyquinoline were inhibito

Catabolite inactivation of isocitrate ly
Catabolite inactivation of isocitrate lyase fromSaccharomyces cerevisiae
✍ Y. S. López-Boado; P. Herrero; S. Gascón; F. Moreno 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 417 KB

A reversible carbon catabolite inactivation step is described for isocitrate lyase from Saccharomyces cerevisiae. This reversible inactivation step of isocitrate lyase is similar to that described for fructose 1,6-bisphosphatase. Addition of 2,4-dinitrophenol, nystatin or glucose to cultures, grown

Identification and characterization of t
Identification and characterization of the genes for two topoisomerase I-interacting proteins fromSaccharomyces cerevisiae
✍ Park, Hangil; Sternglanz, Rolf 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 210 KB 👁 2 views

The two-hybrid system was used to identify yeast genes encoding proteins that interact with DNA topoisomerase I. Two new genes were found and named TOF1 and TOF2. The Tof1 protein has 1238 amino acids and no obvious homologues in databases. The Tof2 protein has 771 amino acids; it is quite closely r