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Regulation of the phosphate (Pi) concentration in UMR 106 osteoblast-like cells: Effect of Pi, Na+ and K+

✍ Scribed by Graham J. Kemp; Hamed I. Khouja; Abdulla Ahmado; R. Graham G. Russell; Alan Bevington

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
825 KB
Volume
11
Category
Article
ISSN
0263-6484

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✦ Synopsis

Osteoblast-like cells possess Na-dependent transporters which accumulate orthophosphate (Pi) from the extracellular medium. This may be important in bone formation. Here we describe parallel measurements of Pi uptake and cellular [Pi] in such cells from the rat (UMR 106-01 and UMR 106-06) and human (OB), and in non-osteoblastic human fibroblasts (Detroit 532 (DET)). In UMR 106-01, cellular [Pi] was weakly dependent on extracellular [Pi] and higher than expected from passive transport alone. [32Pi]-uptake was inhibited by Na deprivation, but paradoxically increased on K deprivation. With Na, 87 per cent of cellular 32P was found in organic phosphorus pools after only 5 min. Na deprivation also decreased cellular [Pi], in both UMR 106-01 and DET, but the decrease was smaller than that in [32Pi]-uptake. Ouabain decreased [32Pi]-uptake and cellular [Pi] in DET, but not in UMR 106-01. Regulation of cellular [Pi] is therefore at least partly dependent on Na/Pi co-transport, but this does not seem to be an exclusive property of osteoblasts.

KEY woaos-Cellular inorganic phosphate; cellular orthophosphate; intracellular metabolism.

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