Regulation of the mdh-sucCDAB operon in Rhizobium leguminosarum
β Scribed by Philip Poole; Colm Reid; Alison K. East; David Allaway; Michael Day; Mary Leonard
- Book ID
- 117519311
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 213 KB
- Volume
- 176
- Category
- Article
- ISSN
- 0378-1097
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RP4-trp hybrid plasmid containing Escherichia coli whole tryptophan operon was conjugatively transferred from E. coli to Rhizobium leguminosarum strains carrying mutations in different trp genes, converting their Trp- phenotype to Trp+. That the phenotype change of the R. leguminosarum cells was due
On the Rhizobium leguminosarum PRE sym plasmid, fixABC and a novel gene fixW were identified upstream of the regulatory gene nifA. The molecular masses of FixABC, 29, 44 and 50 kDa respectively, were estimated by polyacrylamide gel electrophoresis (PAGE) and of FixW, 25 kDa, by PAGE and nucleotide s