Regulation of tetracycline accumulation in Bacillus subtilis bearing B. subtilis plasmid pNS1981
β Scribed by Y. Sumita; K. Shishido
- Book ID
- 109313014
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 239 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0378-1097
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π SIMILAR VOLUMES
The plasmid pKBT1 was derived by in vivo recE4-independent recombinational event(s) yielding a structure containing regions of plasmid and chromosomal origin. BamHI digests of plasmid pUB110 (Kanr/Neor) and Bg/II digests of pTL12 (Tmpr, leuA) were mixed, ligated and used to transform competent cells
The constitutive MLS resistance plasmid pE3692 (2.4 kb) from S. uureus and pE3692-9 plasmid derived from pE3692 by in vitro deletion of the small HindIII-HindIII fragment Stat (0.3 kb) were introduced into S. uureus RN450 and B. subtilis UOT0277 strains. The pE3692 plasmid was completly stable in S.