Regulation of pyrimidine salvage in Aspergillus nidulans: A role for the major regulatory gene areA mediating nitrogen metabolite repression

✍ Scribed by Shaffer, Patricia M. ;Arst, Herbert N.

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 854 KB
• Volume: 198
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00328713

✦ Synopsis

The synthesis of thymine 7-hydroxylase, an alpha-ketoglutarate dependent dioxygenase, is subject both to nitrogen metabolite repression and to oxygen repression, while synthesis of the other pyrimidine salvage pathway dioxygenase, pyrimidine deoxyribonucleoside 2'-hydroxylase, is subject to neither. are A300, an allele of the positive acting regulatory gene are A mediating nitrogen metabolite repression in Aspergillus nidulans, considerably elevates levels of thymine 7-hydroxylase, probably alleviating at least partly both nitrogen metabolite repression and oxygen repression. are A300 has little or no effect on levels of pyrimidine deoxy-ribonucleoside 2'-hydroxylase but does elevate net uptake capacities for thymine, thymidine and deoxyuridine two-fold. are A300 was selected as allowing thymine to supplement a pyrimidine auxotrophy and was found to allow supplementation by thymidine, other pyrimidine nucleosides and pyrimidine salvage intermediates as well. This is the first reported evidence for are A control over an activity(-ies) not directly concerned with nitrogen source utilization.