✦ LIBER ✦

Regulation of myelin basic protein in oligodendrocytes by a soluble neuronal factor

✍ Scribed by Dr. L. Bologa; Y. Aizenman; F. Chiappelli; J. de Vellis

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 583 KB
Volume: 15
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490150409

No coin nor oath required. For personal study only.

✦ Synopsis

Myelin basic protein (MBP) is one of the most important myelin components. Based on our previous shidies, we hypothesized that neurons might have regulatory effects on the production of MBP by oligodendrocytes, and we conducted studies designed to verify this hypothesis. Oligodendroglia-rich cultures from total brain of neonatal rats or mice and pure cultures of embryonic rats or chicks were prepared. Cultures of mouse fibroblasts and astrocytes were prepared as well. We show here that MBP production by oligodendrocytes was greatly enhanced by treatment with either pure neurons, rat neuronal conditioned medium, or chick neuronal conditioned medium, while chemically defined, hormonally supplemented medium or medium conditioned by astroctyes and fibroblasts had no effect on MBP expression. We conclude that the production of MBP by oligodendrocytes is regulated by a nonspecies specific soluble neuronal factor. The conservation of this phenomenon from avian to rodent species implies its critical role in myelination and suggests its potential application as a treatment in demyelination.

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