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Regulation of human Gc (vitamin D — binding) protein levels: Hormonal and cytokine control of gene expression in vitro

✍ Scribed by Chandan Guha; Motoki Osawa; Phillip A. Werner; Robert M. Galbraith; Gary V. Paddock

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 982 KB
Volume: 21
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840210628

No coin nor oath required. For personal study only.

✦ Synopsis

Studies were performed in Hep3B hepatocytes to better elucidate the mechanisms regulating circulating levels of human groupspecific component (Gc). We measured changes in Gc messenger RNA (mRNA) synthesis and levels of secreted protein resulting from treatment of hepatocytes with cytokines and hormones known to influence synthesis of other proteins of hepatic origin. We particularly focused on compounds known to be prototypic stimulants during the acute phase response. Interleukin-6 (IL-6) and dexamethasone were shown to increase Gc mRNA approximately twofold while transforming growth factor beta (TGFP) decreased Gc mRNA in a dose-dependent fashion by up to fivefold. The effects on secreted Gc protein levels were similar. These results indicate that Gc protein appears to be regulated differently than the other members of this gene family, albumin and alpha-fetoprotein (AFP), which are negative acute phase reactants. In addition, these contrasting effects on Gc synthesis of IL-6 and dexamethasone and of TGFP suggest that high basal levels of Gc synthesis may be maintained during the acute phase response. PA-

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