Regulation of growth and morphological modulation of HeLa65 cells in monolayer culture by dibutyryl cyclic AMP, butyrate and their analogs
✍ Scribed by Nimai K. Ghosh; Stephen I. Deutsch; Martin J. Griffin; Rody P. Cox
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 702 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
N6-02'-Dibutyryl adenosine-3',5' monophosphate (DBcAMP) markedly altered the morphology of HeLa cells by increasing average cell size with a n increase in total cell protein and RNA. Such effects were not caused by adenosine 3',5' monophosphate (CAMP) or related nucleosides and nucleotides. Butyrate, an enzyme catalyzed hydrolysis product of DBcAMP, induced a jagged spindle shape in HeLa cells within 8 hours and then caused them to enlarge and resemble those grown with DBcAMP. These effects were specific for butyrate (C,) and pentanoate (C,) and were not observed with isomers, substituted analogs, or other fatty acid derivatives. These morphological effects were prevented by blocking protein synthesis or by altering the cytoskeleton with Colcemid or cvtochalasin B.