Regulation of gene function: A comparison of enzyme activity levels in relation to gene dosage in diploids and triploids ofDrosophila melanogaster

✍ Scribed by John C. Lucchesi; John M. Rawls

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 650 KB
• Volume: 9
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00485589

✦ Synopsis

A study of gene activity in diploid and triploid Drosophila melanogaster females has been petformed. Levels of enzyme activity of X-linked glucose 6-phosphate and 6-phosphogluconate dehydrogenases and autosome-linked ~-glycerophosphate and NADP-dependent isoeitrate dehydrogenases were measured and correlated with DNA content, in crude extracts of whole flies or thoraces. The results show that the contribution of each dose of a given gene to the level of enzyme activity is equal in diploid and tripIoid cells. These findings are discussed in the context of the phenomenon of dosage compensation.