Regulation of Gene Expression

✍ Scribed by Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters

• Publisher: Humana Press
• Year: 2006
• Tongue: English
• Leaves: 316
• Edition: 1
• Category: Library

✦ Synopsis

Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein. Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems.

✦ Table of Contents

_cover......Page 1
00......Page 2
1.0......Page 9
1.1......Page 11
1.2......Page 18
1.3......Page 38
1.4......Page 56
1.5......Page 111
2.0......Page 147
2.1......Page 149
2.2......Page 154
2.3......Page 160
2.4......Page 172
2.5......Page 214
2.6......Page 238
3.0......Page 258
3.1......Page 260
3.2......Page 263
3.3......Page 286
3.4......Page 310
4......Page 312