Regulation of GABAA receptor subunit mRNA expression by the pesticide dieldrin in embryonic brainstem cultures: A quantitative, competitive reverse transcription-polymerase chain reaction study
✍ Scribed by Jiangping Liu; A. Leslie Morrow; Leslie L. Devaud; Dennis R. Grayson; Jean M. Lauder
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 117 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Cyclodiene organochlorine pesticides, such as dieldrin, inhibit ␥-aminobutyric acid (GABA)ergic neurotransmission by blocking the Cl ؊ channel of GABA A receptors. This action may make the developing nervous system especially vulnerable to these neurotoxins, which could interfere with the trophic actions of GABA on developing neurons and alter expression of GABA A receptors. We have used an in vitro model to determine whether exposure to dieldrin alters developmental expression of GABA A receptor subunit mRNA transcripts. Dissociated cell cultures were prepared from embryonic day 14 (E14) brainstem and cultured in serum-containing medium for 1 day in vitro (DIV), then treated for 2 DIV with 10 M dieldrin in serum-free medium. This dose was based on preliminary experiments and previous studies (Nagata et al.: