Regulation of fermentation capacity inSaccharomyces cerevisiae

✍ Scribed by J. P. Dijken; R. Jonker; P. M. Bruinenberg; G. Bwee Houweling-Tan; W. A. Scheffers

• Book ID: 104759672
• Publisher: Springer Netherlands
• Year: 1984
• Tongue: English
• Weight: 126 KB
• Volume: 50
• Category: Article
• ISSN: 0003-6072
• DOI: 10.1007/bf00404924

✦ Synopsis

ary experiments indicate that this sensor may be applicable in short-term experiments on alcohol production by aerated yeast suspensions.

Alcohol oxidase may also be used for colorimetric alcohol assays by an oxidase-peroxidase-ABTS method (cf. Bernt and Lachenicht, 1974). In this case, inactivation or removal of catalase from alcohol oxidase preparations is a prerequisite for a successful application. Since catalase from methanol-grown Hansenula polymorpha can be easily inactivated (Bravo and Wang, 1981), cell-free extracts of this yeast are a suitable source of alcohol oxidase in such colorimetric assays. BERNT, E. and LACHENICHT, R. 1974. Determination in blood, serum, or plasma with automatic analysers (GOD-PERID method), p. 1215-1222. In H. U. Bergmeyer (ed.), Methods of Enzymatic