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Regulation of enzymes in isolated plant nuclei

✍ Scribed by Neeraj Datta; Stanley J. Roux

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
550 KB
Volume
5
Category
Article
ISSN
0265-9247

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✦ Synopsis

Purijied nuclei are being used as a test system to study the regulation of nuclear enzymes in plants. Regulatory agents such as light, hormones and polyamines can stimulate kinases or phosphatases that control nuclear protein phosphorylation and they can modulate the activity of as yet unidentified enzymes required for transcript synthesis andlor stabilization. This essay summarizes current jindings and discusses the advantages and piflalls of using isolated nuclei to investigate how nuclear functions are controlled.

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Investigations on the transfer of isolat
Investigations on the transfer of isolated nuclei into plant protoplasts
✍ H. LΓΆrz; I. Potrykus πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 899 KB

Nuclei were isolated from various types of donor protoplasts and were transferred into receptor protoplasts in numerous combinations. Five percent uptake was achieved under conditions which did not interfere with viability and subsequent culture of receptor protoplasts. Methodological investigations