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Regulation of DNA ligation by the recB protein in Escherichia coli cells

✍ Scribed by Juhani Syväoja; Mauno J. Pyhtilä

Book ID
115917100
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
361 KB
Volume
183
Category
Article
ISSN
0014-5793

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Effect of chloramphenicol and the recB gene product on DNA metabolism in Escherichia coli K12 strains defective in DNA ligase
✍ Morse, Lawrence S. ;Beck, Lawrence A. ;Pauling, Crellin 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 380 KB

We have examined DNA strand breakage, DNA degradation, and the rate of DNA synthesis in lig and lig-recB strains of Escherichia coli K12 incubated in the presence and absence of 3 mug/ml chloramphenicol. Substantial DNA strand breakage and DNA degradation is observed in the lig strain upon growth at