Regulation of cytochrome P450 in a primary culture of rainbow trout hepatocytes
โ Scribed by Marianne D. Sadar; Tommy B. Andersson
- Book ID
- 118729559
- Publisher
- Society for In Vitro Biology
- Year
- 2001
- Tongue
- English
- Weight
- 606 KB
- Volume
- 37
- Category
- Article
- ISSN
- 1475-2689
No coin nor oath required. For personal study only.
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The aim of this study was to test suitable culture conditions for maintaining normal cellular cytoarchitecture and inducibility of P450 enzymes in primary cultures of human hepatocytes by prototypical inducers. The selectivity and sensitivity of a sandwich culture system were determined by treating
Cytochrome P450 2C9 (CYP2C9) expression is regulated by multiple nuclear receptors including the constitutive androstane receptor (CAR) and pregnane X receptor (PXR). We compared coregulation of CYP2C9 with CYP2B6 and CYP3A4, prototypical target genes for human CAR and PXR using human hepatocyte cul