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Regulation of ammonium uptake and metabolism by nitrogen fixing bacteria. III.Clostridium pasteurianum

✍ Scribed by D. Kleiner

Publisher: Springer
Year: 1979
Tongue: English
Weight: 616 KB
Volume: 120
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00423074

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✦ Synopsis

Addition of ammonium salts to N 2 fixing continuous cultures of Clostridium pasteurianum caused immediate stop of nitrogenase synthesis, while the levels of glutamine synthetase, glutamate dehydrogenase and asparagine synthetase remained constant. No evidence for an interconversion of the glutamine synthetase was found. The activities of glutamate synthase in crude extracts were inversely related to the nitrogenase levels. The intracellular glutamine pool rapidly expanded during nitrogenase repression and decreased as fast during derepression while the pool sizes of all other amino acids were not strongly related to the rate of nitrogenase formation. These investigations suggest glutamine as corepressor of nitrogenase synthesis.

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