Regulation of acetate kinase and butyrate kinase by acids in Clostridium acetobutylicum
โ Scribed by Jean Ballongue; Jamel Amine; Henri Petitdemange; Robert Gay
- Book ID
- 109313383
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 432 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0378-1097
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Two metabolic engineering tools, namely gene inactivation and gene overexpression, were employed to examine the effects of two genetic modifications on the fermentation characteristics of Clostridium acetobutylicum. Inactivation of the butyrate kinase gene (buk) was examined using strain PJC4BK, whi
## Abstract Effect of nitrate reduction on fermentation products of __Clostridium perfringens__ was investigated in nutrient media containing glucose. Butyrate, acetate, lactate, and ethanol were produced in the absence of nitrate. Addition of nitrate caused disappearance of butyrate and marked dec
Metabolic flux analysis was used to investigate the roles of the acid formation pathways in Clostridium acetobutylicum. The acid formation pathways were revealed to serve different roles in wildtype fermentations than previously expected. Specifically, enzymes known to catalyze butyrate formation we