Regulation of a Purkinje cell-specific promoter by homeodomain proteins: Repression by Engrailed-2 vs. synergistic activation by Hoxa5 and Hoxb7
✍ Scribed by Sanlioglu, Salih ;Zhang, Xulun ;Baader, Stephan L. ;Oberdick, John
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 294 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
We have previously demonstrated cells. These Hox proteins are shown to synergistically that a short sequence element (L7ATE) within the activate the L7 promoter by cotransfection assay in proximal promoter of a Purkinje cell-specific gene, pcp-2(L7), is required for the normal pattern of exvitro. In contrast, another homeodomain protein that pression of the gene in the cerebellum of transgenic is normally expressed in Purkinje cells only during mice. The presence of a series of TAAT sequence mothe embryonic period, En-2, has a negative effect on tifs in this element suggested its interaction with ho-L7 gene expression. These data suggest a biphasic, meodomain proteins. To extend these observations, combinatorial control mechanism for the Purkinje degenerate oligonucleotides were used to clone by recell-specific expression of the pcp-2(L7) gene. ᭧ 1998 verse-trascriptase polymerase chain reaction mem-