Regulation der Proteophosphomannan-Biosynthese von scp Hefe Candida maltosa H durch Precursorbereitstellung

✍ Scribed by B. Röber; Prof. Dr. G. Reuter

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 780 KB
• Volume: 24
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19840240508

✦ Synopsis

Candida maltosa H and its mutants H3 and H5 differ in their capacity of mannan biosynthesis, but not in their specific activities of phosphomannose isomerase (PMI), phosphomannose mutase and GDP-mannose-pyrophosphorylase.

As sole source of carbon and energy from a mixture of 14C-mannose/glucose or mannose/I4Cglucose, Cmdida maltosa H utilizes both hexoses simultaneously especially under 0,-limit,ation.

With mannose as sole source of carbon and energy the specific activity of PMI increases from 40-90 (aerobic growth) to 550-580 nmolmin-l. mg-l protein (0,-limitation). Phosphoglucose isomerase shows a similar behaviour. PMI purified from glucose-grown cells was found to be inhibited by glyoxylate and fructose-1,6-bisphosphate. The inhibition effect in the biosynthesis of mannose-6-phosphate and its possible role in the regulation of proteophosphomannan biosynthesis are discussed in connection with the affinity of the cell wall for hydrocarbon.