Regulation and characterization of the polarity of cells embedded in a reconstructed basement matrix using a three-dimensional micro-culture system
✍ Scribed by Yu-suke Torisawa; Yuji Nashimoto; Tomoyuki Yasukawa; Hitoshi Shiku; Tomokazu Matsue
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 246 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Three cell lines, that is, the human breast cancer cell line (MCF‐7) and the human mammary epithelial cell line (S‐1) and its malignant form (T4‐2) were embedded in a reconstituted basement membrane (Matrigel) that had 20‐nL pyramid‐shaped silicon microstructures. The proliferative behavior of the MCF‐7 cells was dependent on the surrounding conditions (2‐D, collagen gel, or Matrigel), whereas the respiratory activity of a single cell (F~c~) was almost identical under different culture conditions. The F~c~ value changed with cellular polarity. The F~c~ value for the S‐1 cells was observed to decrease slightly, whereas that of the T4‐2 cells increased 2 days after cultivation in the microstructures within the Matrigel. However, when the T4‐2 cells were cultured in the presence of tyrphostin AG 1478 (T4‐2 tyr) to inhibit epidermal growth factor (EGF) signaling, the F~c~ value decreased slightly and remained almost constant for an additional 1 week; this was similar to the behavior of the S‐1 cells. Further, fluorescence images showed that the T4‐2 tyr cells formed polar structures that were similar to those formed by the S‐1 cells whereas the T4‐2 cells did not form such structures. These results indicate that cellular polarity can be assessed by measuring cellular respiratory activity. Biotechnol. Bioeng. 2007;97: 615–621. © 2006 Wiley Periodicals, Inc.