## Abstract The suppressive role of endogenous regucalcin, which is a regulatory protein of calcium signaling, in the enhancement of nitric oxide (NO) synthase activity in the liver cytosol of rats was investigated. The enzyme activity was measured in a reaction mixture containing either vehicle or
Regucalcin and metabolic disorders: osteoporosis and hyperlipidemia are induced in regucalcin transgenic rats
β Scribed by Masayoshi Yamaguchi
- Book ID
- 106459178
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 671 KB
- Volume
- 341
- Category
- Article
- ISSN
- 0300-8177
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## Abstract The role of regucalcin, which is a regulatory protein in intracellular signaling, in the regulation of Ca^2+^βATPase activity in the mitochondria of brain tissues was investigated. The addition of regucalcin (10^β10^ to 10^β8^ M), which is a physiologic concentration in rat brain tissue
Rats were generated by pronuclear injection of the transgene with a cDNA construct encoding rat regucalcin that is a regulatory protein of Ca2+ signaling. Transgenic (TG) founders were fertile, transmitted the transgene at the expected frequency, and bred to homozygote. Western analysis of the cytos