Regioselectivity in the glutathione-phenylglycidyl ether reaction: 1H NMR characterization of the adduct
✍ Scribed by André De Bruyn; Denis De Keukeleire; Elfride van Den Eeckhout; Willy Baeyens
- Book ID
- 101766043
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2010
- Weight
- 213 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0037-9646
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✦ Synopsis
A detailed 360 MHz ' H NMR analysis leads to the unambiguous structural identification of the compound , which is regioselectivelv formed in the chemical and enzymatical reactions between glutathione and phenvlqlycidyl ether. The thiol group attacks preferentially the least substituted oxirane carbon atom.
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## Abstract The ^1^H NMR spectrum of the macromolecule fraction of rat brain cytosol was investigated following centrifugation and dialysis to remove low molecular weight metabolites and peptides (<3500 daltons). At least seven well resolved resonances were detected between 0.9 and 3.0 ppm in the ^