Regioselective cleavage of di- and poly-ribonucleotides induced by cyclodextrins (cyclomalto-oligosaccharides)
✍ Scribed by Makoto Komiyama
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 376 KB
- Volume
- 192
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Cyclodextrins (cyclomalto-oligosaccharides, CDs) induce regioselective cleavage of the 3',5'-phosphodiester linkages in di-and poly-ribonucleotides at pH 11.08 and 50". 0.15~ Cyclomaltohexaose ((Y-CD) mediates the cleavage of CpA, CpC, CpG, and CpU to give 9697% of cytidine 3'-phosphate together with adenosine, cytidine, guanosine, and uridine, respectively. In the absence of (r-CD, 50-52% of cytidine 2'-phosphate is formed as by-product. (y-CD also promotes the formation of adenosine 3'-phosphate from ApA and ApC, guanosine 3'-phosphate from GpG, and uridine 3'-phosphate from UpU. In contrast, P_CD and yCD enhance the formation of the corresponding 2'-phosphates. Regioselective cleavages of poly [Al, poly [Cl > poly[Gl, and poly[U] are achieved also.