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Regional mapping of 22 microclones around the adenomatous polyposis coli (APC) locus on chromosome 5q

✍ Scribed by Garret M. Hampton; Cathy Howe; Gabriele Leuteritz; Huw Thomas; Walter F. Bodmer; Ellen Solomon; Wolfgang G. Ballhausen

Publisher: Springer
Year: 1991
Tongue: English
Weight: 312 KB
Volume: 88
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00204940

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✦ Synopsis

A previously described genomic library constructed from microdissected DNA has been used to generate a large number of probes around the adenomatous polyposis coli (APC) gene at 5q22. A total of 202 clones were hybridised directly onto a somatic cell hybrid panel containing two APC-related interstitial deletions. Of 75 microclones that gave clear hybridisation signals, 22 independent clones mapped into the region common to both deletions. In addition, 4/22 of the markers are conserved in rodent DNA. These clones should provide a valuable resource for screening cDNA libraries and cloning the DNA around the APC gene in yeast artificial chromosomes.

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