Regional differences in the distribution of myogenic and chondrogenic cells in axolotl limb blastemas

✍ Scribed by Cameron, Jo Ann ;Hinterberger, Timothy J.

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 658 KB
• Volume: 232
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402320214

✦ Synopsis

The distribution of myogenic and chondrogenic cells was determined for axolotl limb blastemas at the medium bud stage, before precartilage condensation occurred. Myogenic potential of midstylopod (upper) and distal-zeugopod (lower) amputation level blastemas, divided into distal, proximal-core, and proximal-peripheral regions, was assayed in vitro. Significantly more myonuclei were observed in upper level proximal-periphery cultures than in ones from any of the other regions. Since cartilage cells give rise almost exclusively to cartilage in the regenerate (Steen, 19681, a heritable cell marker, triploidy, was used to trace the location of chondrogenic cells in histological sections of upper level early and medium bud blastemas. Although there was mixing of the graft cartilage-derived cells and host soft-tissue-derived cells, the previous cartilage cells were found more frequently within the core region at early and medium bud stages. The results of these studies indicate that more chondrogenic cells are present in the core from a very early stage, and that more myogenic cells are present in the proximal periphery by the medium bud stage.