Regional changes in the binding of [3H] concanavalin A to mouse blastocysts at implantation: An autoradiographic study
✍ Scribed by Carollo, J. R. ;Weitlauf, H. M.
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 408 KB
- Volume
- 218
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Implanting and delayed‐implanting mouse blastocysts were incubated in vitro with [^3^H] concanavalin A (Con A), and the distribution of binding on their surfaces was determined by light microscopic autoradiography. The density of binding was uniform on the trophectoderm of delayed‐implanting embryos and was not changed on the polar surface of implanting embryos. However, binding was reduced on the proximal mural and distal mural trophectoderm of implanting blastocysts by 36% and 60%, respectively. These results suggest that there is a regional reduction in the density of mannose‐like sugars on the surface of mouse blastocysts at the time of attachment and implantation.