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Regional assignment of 41 human DNA fragments on chromosome 7 by means of a somatic cell hybrid panel

✍ Scribed by Anke Jobs; Dirk Klein-Bölting; Almuth-Sigrun Jandel; Albert Driesel; Klaus Olek; Karl-Heinz Grzeschik

Publisher
Springer
Year
1990
Tongue
English
Weight
458 KB
Volume
84
Category
Article
ISSN
0340-6717

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✦ Synopsis

To detect new restriction fragment length polymorphisms that would cover human chromosome 7 with a network of genetic landmarks, a chromosome 7-specific phage gene library was screened for human single-copy fragments. With use of a somatic cell hybrid panel containing defined regions of human chromosome 7, 41 cloned human single-copy sequences were assigned to five regions of this chromosome. Of special importance are the cell hybrid clones GM1059Rag5 and 7851Rag10-1, derived from human cells with interstitial deletions spanning the bands 7q22-q32, within which the cystic fibrosis gene is located. Twelve new probes are described in 7q22-q32, five of which detect a total of six RFLPs.

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Characterization of a set of X-linked se
Characterization of a set of X-linked sequences and of a panel of somatic cell hybrids useful for the regional mapping of the human X chromosome
✍ I. Oberlé; G. Camerino; C. Kloepfer; J. P. Moisan; K. H. Grzeschik; B. Hellkuhl; 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 1005 KB

We have characterized 19 DNA fragments originating from the human X chromosome. Most of them have been isolated from an X chromosome genomic library (Davies et al. 1981) using a systematic screening procedure. These DNA probes have been used to search for restriction fragment length polymorphisms (R