Region-specific expression of mario reveals pivotal function of the anterior nondigit region on digit formation in chick wing bud
✍ Scribed by Takanori Amano; Koji Tamura
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 512 KB
- Volume
- 233
- Category
- Article
- ISSN
- 1058-8388
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
We report the region‐specific expression of a novel gene, named mario, whose expression domain is in the distal tip of the presumptive and developing digit 2 region in the developing chick wing bud. The anterior region‐specific expression of mario corresponds well with the presence of digit 2, and fate map analysis showed that mario expression at early stages represents the presumptive digit 2 region. Using mario expression as a region‐specific marker for the digit 2 region, several surgical operations were performed to obtain insights into digit 2 development in the chick wing. Cell fate tracing concomitant with a zone of polarizing activity (ZPA) implantation revealed that an additional digit 2 in the ZPA implantation into the anterior or middle region of wing bud is derived from the original digit 2 region (mario‐positive region). Surgical manipulations revealed that the anterior nondigit region has an inhibitory effect on digit 2 formation. Taken together, these results suggest that the most‐anterior region, including the anterior necrotic zone, restricts the position of digit 2 region by limiting the anterior border of the digit 2 region and preventing its expansion. Developmental Dynamics 233:326–336, 2005. © 2005 Wiley‐Liss, Inc.