Regeneration ofGossypium hirsutumandG. barbadensefrom shoot apex tissues for transformation
✍ Scribed by Jean Gould; Sharon Banister; Osamu Hasegawa; Michal Fahima; Roberta H. Smith
- Book ID
- 104675310
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 822 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
✦ Synopsis
A method of regenerating cotton plants from the shoot apical meristem of seedlings was developed for use with particle gun and Agrobacterium-mediated transformation. This method was developed to circumvent the problems of genotype restriction and chromosomal damage frequently encountered in cotton regeneration in tissue culture through somatic embryogenesis. In this procedure, the cells of the shoot meristem are targeted for transformation. Normal and fertile plants of Gossypium barbadense Pima S-6, and 19 cultivars of G. hirsutum were regenerated using this method. Shoot regeneration from these tissues was direct and relatively rapid. A MS based, hormone-free medium could be used with all the varieties tested.