Regeneration ofElaeagnus angustifoliafrom leaf segments of in vitro-derived shoots

✍ Scribed by Athanasios S. Economou; Eleni M. Maloupa

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 297 KB
• Volume: 40
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00048135

✦ Synopsis

Shoot regeneration was achieved from in vitro-produced leaves of Elaeagnus angustifolia L. Half-leaf explants from the terminal part of the shoot produced more shoots than explants from the basal part of the in vitro-derived shoots on agar-solidified WPM medium supplemented with 1 laM benzyladenine (BA). In liquid medium of the same formulation, compact shoots that did not elongate were formed on the explants. Leaf cross-section explants (1 mm thick) produced shoots both on solid and liquid medium with 1 ~ BA, whereas again compact shoots were formed with 10 ~tM BA. Further shoot development on these explants was promoted by their transfer to fresh solid medium containing 1 ~tM BA and 1 lxM gibberellic acid (GA3).