Regeneration of shoots from pea (Pisum sativum) hypocotyl explants
β Scribed by S. V. S. Nielsen; G. B. Poulsen; M. E. Larsen
- Book ID
- 110900342
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 712 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0031-9317
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Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures
A procedure has been developed which allows high frequency adventitious shoot regeneration from immature cotyledons of pea. Prolific shoot regeneration occurred following an initial callus growth on a Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 4 mg/l Ξ±-naphthal