Regeneration of plantlets from hypocotyl-derived callus ofCoptis teeta

✍ Scribed by Pramod Tandon; Tilok S Rathore

• Book ID: 104618345
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 464 KB
• Volume: 28
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00039923

✦ Synopsis

Callus cultures of Coptis teeta were established from hypocotyl segments (excised from aseptically germinating seeds) on Murashige and Skoog (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin. Microshoots were produced within 6-7 weeks of subculturing this callus in 1/2 MS nutrient medium supplemented with kinetin alone. Excised microshoots were rooted in 1/2 MS nutrient medium containing indolebutyric acid (IBA). The complete plantlets were hardened and established.