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Regeneration of albino plantlets from suspension culture derived protoplasts ofPoa pratensisL. (Kentucky bluegrass)

โœ Scribed by P. van der Valk; M. A. C. M. Zaal; J. Creemers-Molenaar

Publisher
Springer
Year
1988
Tongue
English
Weight
610 KB
Volume
39
Category
Article
ISSN
0014-2336

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โœฆ Synopsis

A procedure for the regeneration of callus and albino plantlets from protoplasts of Kentucky bluegrass (Poa pratensis L .), cultivar Kimono, is described . Protoplasts were isolated from a suspension culture with shoot-forming ability, which had been initiated from immature inflorescence derived callus . Protoplasts were cultured in modified Kao & Michayluk (KM8P) liquid medium . Plantlets were recovered from protoplast-derived callus growing on hormone-free modified Murashige & Skoog (MS) medium . While the plantlets derived from the original callus cultures were green, those from suspension cultures and suspension culture derived protoplasts were albino . Abbreviations : 2,4-D -2,4-dichlorophenoxyacetic acid, AA -Amino acid medium (Muller & Grafe, 1978), MS -Murashige & Skoog (1962) medium, KM8P -Kao & Michayluk (1975) medium, MES -2-(N-Morpholino)ethane sulfonic acid, N6 -medium of Chu et al . (1975) .

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