Regeneration in cultures ofPapaver bracteatumas influenced by growth hormones and temperature

✍ Scribed by Ihsan Ilahi; E. G. Ghauri

• Book ID: 104616097
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 254 KB
• Volume: 38
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00034450

✦ Synopsis

Callus was induced on Papaver bracteatum Lindl. seedlings inoculated on Murashige & Skoog (1962) medium supplemented with a-naphthaleneacetic acid (NAA) (1.0 mg 1 -l ) and benzylamine purine (BA) (0.5 mg l-l). Subculture resulted in excellent callus proliferation but no organogenesis. Shoots were regenerated in cultures grown on MS medium containing NAA (1.0 mg 1-1), BA (0.5 mg 1 -l and casein hyrdrolyzate (2.0 mg I-1). The shoots developed into plantlets after 8 weeks of culture, and were induced to root on 1/2 MS without the addition of growth regulators. The rooted plantlets were transferred to soil after hardening.

MS at full strength was found inhibitory for callus induction and proliferation, but 1/2 MS was suitable. Similarly callus growth was very slow at 25°C but increased when the temperature was lowered to 15°C as did bud initiation.