Regeneration from long-term cell suspension cultures of tepary bean (Phaseolus acutifolius)
โ Scribed by A. S. Kumar; O. L. Gamborg; M. W. Nabors
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 794 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0721-7714
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Cell suspensions have been used for obtaining somaclonal variability and for the production of secondary metabolites, such as aroma compounds, natural dyes and drugs. Undifferentiated growing cells completely lose their regeneration capacity due to the formation of cells with abnormal DNA content du
Suspension cultures which maintained embryogenic potency for more than 18 months were established from excised immature embryos of rice (Oryza sativa L. cv. Konansou). The cultures were subcultured every three days in N6 medium supplemented with proline (10 mM), casein hydrolysate (300 mg/l), sucros