Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography

Refolding of therapeutic proteins produc

Refolding of therapeutic proteins produced in Escherichia coli as inclusion bodies

✍ Satoru Misawa; Izumi Kumagai 📂 Article 📅 1999 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 190 KB

Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclus