We have used a cellulose acetate, hollow-fibre (HF) ultrafiltration membrane to refold bovine carbonic anhydrase, loaded into the lumen space, by removing the denaturant through controlled dialysis via the shell side space. When challenged with GdnHCl-denatured carbonic anhydrase, 70% of the loaded
Refolding of a membrane protein in a microfluidics reactor
โ Scribed by Nathan R. Zaccai; Kamran Yunus; S. M. Matthews; Adrian C. Fisher; Robert J. Falconer
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 272 KB
- Volume
- 36
- Category
- Article
- ISSN
- 1432-1017
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