Refining a proximal breakpoint cluster at chromosome 3p11.2 in non-papillary renal cell carcinomas

✍ Scribed by Peter Bugert; Christiane Kenck; Mónica Wilhelm; Gyula Kovacs

• Book ID: 102654682
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 525 KB
• Volume: 68
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19961211)68:6<723::aid-ijc5>3.0.co;2-5

✦ Synopsis

Our previous cytogenetic and microsatellite analyses showed a terminal deletion of chromosome 3p segments with a breakpoint region between 3plI.2 and 3~14.1 bands in 98% of non-papillary renal cell carcinomas (RCC). This breakpoint region covers approx. 20-25 cM genetic distance. Earlier, we found a higher frequency of rearrangements at chromosome 3pll.2 and have therefore now analyzed 74 sporadic and 26 VHL-associated RCCs with 6 polymorphic microsatellite markers mapped to this region. Thirty-three per cent of the breakpoints were mapped to a genetic distance of less than onetwentieth of the large breakpoint region 3pl1.2-14.1. We suggest that unstable DNA sequences at chromosome 3p I I .2 sewe as a genetic basis for deletions and homologous and non-homologous recombinations to remove distal 3p sequences with one copy of RCC gene(s) in different types of tumors. We have identified a YAC clone 909d5 that spans the most frequently occurring breaks between loci D3S I25 I, 035 I 10 I, and D3S I552 and allows this region to be cloned.