✦ LIBER ✦

Reexpression of HPRT activity following cell fusion with polyethylene glycol

✍ Scribed by Bohdan Bakay; Marsha Graf; Sharen Carey; Erkki Nissinen; William L. Nyhan

Publisher: Springer
Year: 1978
Tongue: English
Weight: 538 KB
Volume: 16
Category: Article
ISSN: 0006-2928
DOI: 10.1007/bf00484080

No coin nor oath required. For personal study only.

✦ Synopsis

Polyethylene glycol-1000 (PEG-1000) induced fusion of HPRT (E.C. 2.4.2.8) deficient Chinese hamster cells with alpha-galactosidase A (E.C. 2.3.1.22) deficient cells from a patient with Fabry's disease yielded hybrids which contained both human and hamster HPRT, G6PD (E.C. 1.1.1.49), and APRT (E.C. 2.4.2.7) and Chinese hamster alpha-galactosidase B. Thus PEG-1000 mediated somatic cell fusion led to reexpression of Chinese hamster HPRT. It did not restore the expression of human alpha-galactosidase. Since PEG-1000 treatment of HPRT- Chinese hamster cells in the absence of human cells yielded no HPRT+ cells, it is concluded that the element responsible for the restoration of rodent HPRT was contributed by the human cells and not by the agent employed to promote fusion.

📜 SIMILAR VOLUMES

Formation of tetraploid mouse blastocyst

Formation of tetraploid mouse blastocysts following blastomere fusion with polyethylene glycol

✍ Eglitis, Martin A. 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 655 KB

## Abstract A rapid method to produce tetraploid embryos by blastomere fusion using polyethylene glycol (PEG) has been developed. Individual four‐cell stage blastomeres were aggregated into pairs with phytohemagglutinin (PHA) and then treated with 45% (w/v) PEG (MW 1,000). 56.8% (804/1,416) of trea

Fusion of mammalian somatic cells with p

Fusion of mammalian somatic cells with polyethylene glycol 400 MW

✍ Mike A. Clark; Andrew H. Crenshaw; Jerry W. Shay 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 281 KB

VP22-mediated intercellular transport co

VP22-mediated intercellular transport correlates with enhanced biological activity of MybEngrailed but not (HSV-I) thymidine kinase fusion proteins in primary vascular cells following non-viral transfection

✍ Paul J. Sheridan; Allan Lawrie; David C. Crossman; Cathy M. Holt; Christopher M. 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 311 KB

## Abstract ## Background The intercellular transport properties of the herpes simplex virus (HSV) protein VP22 have been harnessed to enhance the effectiveness of viral gene transfer. We investigated the intercellular transport and biological effects of VP22 fused with the dominant negative c‐Myb