Important intrinsic characteristics of the rotating frame nuclear Overhauser effect spectroscopy (ROESY) experiment were found to be advantageous in DNA solution structure determination. In a ROESY experiment, the different mechanisms of relaxation result in different signs of cross peaks, enabling
Reevaluation of the Off-Resonance Rotating-Frame Nuclear Overhauser Effect (O-ROESY) Experiment
β Scribed by Douglas Brooks; Patrick Ladam; Kazuo Kuwata; Thomas Schleich
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 110 KB
- Volume
- 117
- Category
- Article
- ISSN
- 1064-1858
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15N off-resonance rotating frame relaxation can be applied to the study of internal dynamics in proteins in the millisecond to microsecond regime. We show that the performance of existing methods can be improved by application of simultaneous amplitude and phase-modulated adiabatic RF pulses to alig
The trisaccharide beta-D-Glcp-(1 --> 2)[beta-D-Glcp-(1 --> 3)]alpha-D-Manp-OMe, a model for branching regions in oligosaccharides, has been investigated by one-dimensional DPFGSE (1)H, (1)H nuclear Overhauser effect spectroscopy (NOESY) and transverse rotating-frame Overhauser effect spectroscopy (T