Reduction of syndecan-1 mRNA in cervical-carcinoma cells is involved with the 3′ untranslated region

✍ Scribed by Kazuyoshi Nakanishi; Naohisa Yoshioka; Kiyomasa Oka; Akira Hakura

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: French
• Weight: 147 KB
• Volume: 80
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19990209)80:4<527::aid-ijc8>3.0.co;2-y

✦ Synopsis

Syndecan-1 is a transmembrane proteoglycan expressed predominantly in epithelial cells. Studies with immunohistochemistry have shown that syndecan-1 expression is reduced in carcinoma derived from human epidermis. Here we show that syndecan-1 mRNA, which is abundant in human primary keratinocyte (HK) and HaCaT spontaneous immortalized keratinocyte, is decreased in cervical-carcinoma cell lines. Further, in relation to a long and well-conserved 3Ј untranslated region (3Ј UTR) of syndecan-1 cDNA, we examined whether 3Ј UTR is involved with syndecan-1-mRNA reduction in cervical-carcinoma cells. A stable transfection experiment showed that addition of the 3Ј UTR does not affect expression in HaCaT, but that syndecan-1 cDNA containing the 3Ј UTR is not expressed efficiently selectively in cervicalcarcinoma cell lines. The transient assay with CAT reporter plasmids linking the 3Ј UTR confirmed this, and indicated that the 3Ј end of the 3Ј UTR (nt 2285-2410) is required to influence expression in cervical-carcinoma cells. Further excessive expression of syndecan-1 suppressed growth in cervical-carcinoma cells. These results demonstrate that the reduction of syndecan-1 mRNA involved with the 3Ј untranslated region gives growth advantage to cervical-carcinoma cells. Int.