✦ LIBER ✦

Reduction of RNA in single cell protein in conjunction with fiber formation

✍ Scribed by William H. Daly; Leonard P. Ruiz

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 130 KB
Volume: 16
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260160211

No coin nor oath required. For personal study only.

✦ Synopsis

Reduction of RNA in Single Cell Protein in Conjunction with Fiber Formation

Two of the fundamental problems facing the commercialization of single cell protein (SCP) are protein fabrication and removal of the nucleic acids. The recent reports by Hedenskog et al.l.* confirm our earlier observation3 that selective hydrolysis of nucleic acids to low molecular weight, dialyzable nucleotides in alkali is an effective method for reducing the nucleic acid content. Since the SCP spinning processes described by HBden4 and Rha5 require that the protein be dispersed in alkaline media, selection of a suitable digestion time to effect hydrolysis prior to spinning should degrade the RNA to fragments which remain soluble in the coagulating bath. Indeed, this communication describes a simple one-step method for spinning SCP with concurrent removal of 90% of the RNA. The procedure does not require a high temperature alkali treatment; this minimizes the possibility of base catalyzed toxic by-product formation.6

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