✦ LIBER ✦

Reduction of plant tissue nitrate to nitric oxide for mass spectrometric 15N analysis

✍ Scribed by R.J. Volk; C.J. Pearson; W.A. Jackson

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 409 KB
Volume: 97
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90336-1

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✦ Synopsis

A procedure based on that of Van Slyke and LoMonte (Microchem.

J. 14,[608][609][610][611][612][613][614][615][616][617][618][619][620][621][622][623][624][625][626] 1969) is described for the mass spectrometric analysis of 15N in nitrate extracted from plant tissue. The extract is evaporated to dryness in a disposable borosilicate test tube, which forms the basal part of a reaction flask. After evacuation of the flask, nitrate is reduced to nitric oxide by sonication with mercury in 18 N HZSOI. The atom percentage 15N is calculated from the relative intensities of the 14N0 and 15N0 peaks of the mass spectrum. The method is unaffected by reduced nitrogen compounds which are present in plant tissue extracts.

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✍ I. Sich; R. Russow 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 77 KB 👁 2 views

Nitric oxide (NO) and nitrous oxide (N 2 O) are two important trace gases in the atmosphere. Determining the concentration and 15 N abundance of NO and N 2 O in air is difficult owing to their very low concentration in the atmosphere (NO `1 ppb(v); N 2 O % 0.32 ppm(v)). Although 15 N analysis of N 2