Reduction of hippocampal N-acetyl aspartate level in aged APPSwe/PS1dE9 transgenic mice is associated with degeneration of CA3 pyramidal neurons
✍ Scribed by Wei Xu; Yanqiang Zhan; Wei Huang; Xuxia Wang; Sumin Zhang; Hao Lei
- Book ID
- 102384640
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 387 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
Age‐related metabolic changes in the hippocampus of APP~Swe~/PS1~dE9~ mice were measured with long echo‐time in vivo ^1^H‐magnetic resonance spectroscopy (^1^H‐MRS). Thioflavine S staining and Nissl staining were used to characterize deposition of Aβ aggregates and neuronal degeneration in the transgenic animals, respectively. The results showed that the APP~Swe~/PS1~dE9~ mice had significantly decreased hippocampal N‐acetyl aspartate (NAA)/total creatine (tCr) level at 16 months of age, which was associated with degeneration of and intracellular deposition of thioflavine S‐positive materials in hippocampal CA3 pyramidal neurons. The results of this study provide direct evidence showing association among Aβ pathology (intracellular deposition of thioflavine S‐positive materials), neuronal degeneration, and metabolic changes observable with in vivo ^1^H‐MRS in the hippocampus of APP~Swe~/PS1~dE9~ mice. © 2010 Wiley‐Liss, Inc.