The NADH: (acceptor) oxidoreductase (EC 1.6.99.3) was isolated from human erythrocyte ghosts by a procedure including Triton X-100 solubilization, affinity chromatography on an NAD+-Sepharose 4B column, ammonium sulfate precipitation, and isoelectric focusing. This enzyme preparation was characteriz
โฆ LIBER โฆ
Reduction of extracellular potassium ferricyanide by transmembrane NADH: (Acceptor) oxidoreductase of human erythrocytes
โ Scribed by W. Schipfer; B. Neophytou; R. Trobisch; O. Groiss; H. Goldenberg
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 412 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0020-711X
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Suspension-cultured cells of sugarcane (Saccharurn sp. hybrids) did not oxidize exogenously supplied NADH in the absence of ferricyanide (potassium hexacyanoferrate [III]), whereas they did at a low rate in the presence of ferricyanide. Concomitantly, ferricyanide was reduced at a slow rate. Neither