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Reduction and alkylation of proteins in preparation of two-dimensional map analysis: Why, when, and how?

✍ Scribed by Ben Herbert; Marina Galvani; Mahmoud Hamdan; Erna Olivieri; John MacCarthy; Sanne Pedersen; Pier Giorgio Righetti


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
473 KB
Volume
22
Category
Article
ISSN
0173-0835

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Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the