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Reducing the background to silver-stained polyacrylamide gels after two-dimensional electrophoresis

✍ Scribed by Robert Amess; Dr. S. Peter Spragg


Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
328 KB
Volume
7
Category
Article
ISSN
0173-0835

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We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m